bbsi digested px458 wtcas9 plasmid (Addgene inc)
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Addgene inc
bbsi digested px458 wtcas9 plasmid
Bbsi Digested Px458 Wtcas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+digested+px458+wtcas9+plasmid/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm39910618-236-18-23
Average 96 stars, based on 4114 article reviews
Bbsi Digested Px458 Wtcas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 4114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbsi+digested+px458+wtcas9+plasmid/pSpCas9(BB)-2A-GFP+(PX458)+(Plasmid+%2348138)/pm39910618-236-18-23
Average 96 stars, based on 4114 article reviews
bbsi digested px458 wtcas9 plasmid - by Bioz Stars,
2026-10
96/100 stars
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Subcloning:Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation Article Snippet: The ERT2-Cre-ERT2 transgene was PCR amplified (Additional file : Table 1) from (Addgene #13,777) into the pSicoR lentiviral back bone containing a t2a-blasticidin selection cassette via In-Fusion reaction (Takara Bio Inc.) following SrfI/NheI digestion of the parent vector and blunting (NEB Quick-blunting). .. Nsd1 ablation in MEFs was performed following cohesive-end subcloning of the annealed sgNsd1 (see gene targeting methods) into Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation. Article Snippet: The ERT2-Cre-ERT2 transgene was PCR amplified (Additional file 2: Table 1) from (Addgene #13,777) into the pSicoR lentiviral back bone containing a t2a-blasticidin selection cassette via In-Fusion reaction (Takara Bio Inc.) following SrfI/NheI digestion of the parent vector and blunting (NEB Quick-blunting). .. Nsd1 ablation in MEFs was performed following cohesive-end subcloning of the annealed sgNsd1 (see gene targeting methods) into Plasmid Preparation:Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation Article Snippet: The ERT2-Cre-ERT2 transgene was PCR amplified (Additional file : Table 1) from (Addgene #13,777) into the pSicoR lentiviral back bone containing a t2a-blasticidin selection cassette via In-Fusion reaction (Takara Bio Inc.) following SrfI/NheI digestion of the parent vector and blunting (NEB Quick-blunting). .. Nsd1 ablation in MEFs was performed following cohesive-end subcloning of the annealed sgNsd1 (see gene targeting methods) into Article Title: SETD2 loss-of-function uniquely sensitizes cells to epigenetic targeting of NSD1-directed H3K36 methylation. Article Snippet: The ERT2-Cre-ERT2 transgene was PCR amplified (Additional file 2: Table 1) from (Addgene #13,777) into the pSicoR lentiviral back bone containing a t2a-blasticidin selection cassette via In-Fusion reaction (Takara Bio Inc.) following SrfI/NheI digestion of the parent vector and blunting (NEB Quick-blunting). .. Nsd1 ablation in MEFs was performed following cohesive-end subcloning of the annealed sgNsd1 (see gene targeting methods) into |